diff --git a/test/functional/mempool_accept.py b/test/functional/mempool_accept.py
index 27ecc3b4a84..2155b8de6b1 100755
--- a/test/functional/mempool_accept.py
+++ b/test/functional/mempool_accept.py
@@ -9,6 +9,10 @@ from decimal import Decimal
 import math
 
 from test_framework.test_framework import BitcoinTestFramework
+from test_framework.mempool_util import (
+    DEFAULT_MIN_RELAY_TX_FEE,
+    DEFAULT_INCREMENTAL_RELAY_FEE,
+)
 from test_framework.messages import (
     MAX_BIP125_RBF_SEQUENCE,
     COIN,
@@ -81,6 +85,11 @@ class MempoolAcceptanceTest(BitcoinTestFramework):
         assert_equal(node.getblockcount(), 200)
         assert_equal(node.getmempoolinfo()['size'], self.mempool_size)
 
+        self.log.info("Check default settings")
+        # Settings are listed in BTC/kvB
+        assert_equal(node.getmempoolinfo()['minrelaytxfee'], Decimal(DEFAULT_MIN_RELAY_TX_FEE) / COIN)
+        assert_equal(node.getmempoolinfo()['incrementalrelayfee'], Decimal(DEFAULT_INCREMENTAL_RELAY_FEE) / COIN)
+
         self.log.info('Should not accept garbage to testmempoolaccept')
         assert_raises_rpc_error(-3, 'JSON value of type string is not of expected type array', lambda: node.testmempoolaccept(rawtxs='ff00baar'))
         assert_raises_rpc_error(-8, 'Array must contain between 1 and 25 transactions.', lambda: node.testmempoolaccept(rawtxs=['ff22']*26))
diff --git a/test/functional/test_framework/mempool_util.py b/test/functional/test_framework/mempool_util.py
index 0e9c821e2ea..1587c82e2f8 100644
--- a/test/functional/test_framework/mempool_util.py
+++ b/test/functional/test_framework/mempool_util.py
@@ -20,6 +20,10 @@ from .wallet import (
 )
 
 ORPHAN_TX_EXPIRE_TIME = 1200
+# Default for -minrelaytxfee in sat/kvB
+DEFAULT_MIN_RELAY_TX_FEE = 1000
+# Default for -incrementalrelayfee in sat/kvB
+DEFAULT_INCREMENTAL_RELAY_FEE = 1000
 
 def assert_mempool_contents(test_framework, node, expected=None, sync=True):
     """Assert that all transactions in expected are in the mempool,